BIOACTIVE WATER NAFTUSSYA AND OZOKERITE HAVE THE SAME NEURO- ENDOCRINE-IMMUNE EFFECTS IN MALE RATS CAUSED BY ARYL HYDROCARBONS

Abstract

Background. It is known that biological activity of curative water Naftussya (N) spa Truskavets’ (Ukraine) caused by its organic substances, genetically connected with Oil and Ozokerite (O) from Boryslav’s layer, located near from Truskavets’. Because probable aryl hydrocarbon receptor (AhR) agonists are present in both N and O, and AhR-expressing macrophages are localized in lymphoid tissue associated with both the gut (GALT) and the skin (SALT), we hypothesized that both oral use of N, and application to the skin O can have similar immunotropic effects. The study is devoted to testing this hypothesis. Material and Methods. Experiment was performed on 50 healthy Wistar male rats. Animals of the first group remained intact, using tap water. Instead, the other rats received the same tap water and N through the tube for 6 days. Another group received together with N three applications of O, and the last - only O. The day after the completion of the drinking/application course we recorded the parameters of the neuro-endocrine-immune complex. Results. Discriminant analysis revealed 23 parameters (5 of the thymus, 3 of the spleen, 6 of blood, 7 endocrine and 2 of HRV), the set of which intact, control and experimental rats differ significantly from each other. Calculation of centroids separately for groups treated with N, O, or both balneofactors showed no differences at all. Conclusion. The modulating effect on 23 parameters of the neuro-endocrine-immune complex N and O, as well as their combination is almost equivalent which is probably due to the binding of aryl hydrocarbons to AhR of macrophages of GALT and SALT.